Phosphorylation of smg p21B in rat peritoneal mast cells in association with histamine release inhibition by dibutyryl‐cAMP

FEBS Letters

Izushi, Keiji¹  Tasaka, Kenji¹  Shirasaka, Taihei¹  Chokki, Manabu¹ 

[1] Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700, Japan

关键词: smg p21B;    Protein phosphorylation;    Protein kinase A;    Histamine release;    Rat mast cell;    protein kinase A;    cyclic AMP-dependent protein kinase;    protein kinase C;    Ca2+/phospholipid-dependent protein kinase;    G protein;    GTP-binding protein;    Bt2cAMP;    N 6;    O 2′-dibutyryl cyclic AMP;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    PMSF;    phenylmethylsulfonyl flouride;    GDS;    GDP dissociation stimulator;    IP3;    inositol 1;    4;    5-trisphosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PGE1;    prostaglandin E1;   

DOI  :  10.1016/0014-5793(92)81480-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

IP₃ formation and histamine release from rat peritoneal mast cells stimulated by compound 48/80 were dose-dependently inhibited by Bt₂cAMP. These inhibitions were restored to the control level in the presence of H-8, a protein kinase A inhibitor. The 22 kDa protein in mast cells was revealed as a markedly phosphorylated protein by incubating with Bt₂cAMP, and this phosphorylation was also diminished by H-8. The 22 kDa phosphoprotein of rat mast cells comigrated with phosphorylated smg p21B, purified from human platelets and phosphorylated by protein kinase A in cell-free system, in both one- and two-dimensional PAGE analysis. Moreover, 22 kDa protein in mast cells was identified as smg p21B by immunoblot analysis using an antibody against smg p21B. From the present study, it became clear that smg p21B is phosphorylated by means or protein kinase A system in rat peritoneal mast cells, and it was assumed that phosphorylated smg p21B plays some important role in the suppression of IP₃ formation and histamine release from rat peritoneal mast cells.

【 授权许可】

Unknown