Molecular cloning of G protein α subunits from the central nervous system of the mollusc Lymnaea stagnalis

FEBS Letters

Vreugdenhil, Erno¹  Weidemann, Wolfgang¹  Planta, Rudi J.¹  Knol, Jaco C.¹  van Heerikhuizen, Harm¹ 

[1] Department of Biochemistry and Molecular Biology, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands

关键词: G protein α subunit;    Central nervous system;    cDNA cloning;    Lymnaea stagnalis;   

DOI  :  10.1016/0014-5793(92)81474-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The central nervous system of the pond snail, Lymnaea stagnalis, contains many large, identified neurons which can be easily manipulated making it an advantageous model system to elucidate in vivo the architecture of neuronal signal transduction pathways. We have isolated three cDNA clones encoding G protein α subunits that are expressed in the Lymnaea CNS, i.e. Gα_o, Gα, and Gα_i. The deduced proteins exhibit a very high degree of sequence identity to their vertebrate and invertebrate counterparts. The strong conservation or G protein α subunits suggests that functional insights into G protein-mediated signalling routes obtained through the experimental amenability of the Lymnaea CNS will have relevance for similar pathways in the mammalian brain.

【 授权许可】

Unknown