Structure of UvrABC excinuclease—UV‐damaged DNA complexes studied by flow linear dichroism DNA curved by UvrB and UvrC

FEBS Letters

Nordén, Bengt²  Fuchs, Robert P.P.¹  Bertrand-Burggraf, Elisabeth¹  Takahashi, Masayuki¹ 

[1] Groupe de Cancérogénèse et de Mutagénèse Moléculaire et Structurale, Institut de Biologie Moléculaire et Cellulaire du CNRS, 15 rue René Descartes, F-67084 Strasbourg Cedex, France;Department of Physical Chemistry, Chalmers University of Technology, S-41296 Gothenburg, Sweden

关键词: UvrABC excinuclease;    Linear dichroism;    Protein—DNA complex;    UV damaged DNA;    DNA repair;    UV;    ultraviolet light;    LD;    linear dichroism;   

DOI  :  10.1016/0014-5793(92)81448-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction between UvrABC excinuclease from Escherichia coli and ultraviolet light-(UV) damaged DNA was studied by flow linear dichroism. The dichroism signal from DNA was drastically decreased in intensity upon incubation with UvrA and UvrB or whole enzyme in the presence off effector ATP. The change was specific for UV-damaged DNA, and a concluded suppressed DNA orientation suggests the wrapping of DNA around the protein. The incubation with the UvrC subunit alone also somewhat reduces the signal, however, in this case the change was smaller and not specific for UV-damaged DNA. The structural modification of DNA, promoted by the (UvrA₂-UvrB) complex probably facilitates or stabilizes the interaction of the UvrC subunit with DNA for the excision.

【 授权许可】

Unknown