m‐Iodobenzylguanidine increases the mitochondrial Ca2+ pool in isolated hepatocytes

FEBS Letters

Orrenius, Sten¹  Kass, George E.N.¹  Juedes, Marlene J.¹ 

[1] Department of Toxicology, Karolinska Institutet, Box 60400, S-10401, Stockholm, Sweden

关键词: m-Iodobenzylguanidine;    Mitochondria;    Ca2+ efflux;    Hepatocyte;    ADP-ribosylation;    2;    5-Di-(tert-butyl)-1;    4-hydroquinone;    [Ca2+]i;    cytosolic free Ca2+ concentration;    MIBG;    m-iodobenzylguanidine;    FCCP;    carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone;    tBuHQ;    2;    5-di(tert-butyl)-1;    4-hydroquinone;    arsenazo III;    2;    2′-[1;    8-dihydroxy-3;    6-disulfo-2;    7-naphthalene-bis(azo)]dibenzenearsonic acid;    fluo 3;    1-(2-amino-5-(2;    7-dichloro-6hydroxy-3-oxy-9-9-xanthenyl)-phenoxy)-2-(2-amino-5-methylphenoxy)ethane-N;    N;    N′;    N′-tetraacetic acid;    AM;    acetoxymethyl ester;    MPP+;    1-methyl-4-phenylpyridine;   

DOI  :  10.1016/0014-5793(92)81179-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The incubation of isolated hepatocytes with the inhibitor of protein mono ADP-ribosylation, m-iodobenzylguanidine (MIBG), resulted in an increase in the size of the mitochondrial Ca²⁺ pool, without alteration of the non-mitochondrial Ca²⁺ store(s). This increase was abolished when the cytosolic free Ca²⁺ concentration ([Ca²⁺]_i) was buffered by prior loading of the cells with fluo 3. Elevating [Ca²⁺]_i by releasing the endoplasmic reticular Ca²⁺ store with 2,5-di-(tert-butyl)-1,4-hydroquinone resulted in a synergistic increase in the magnitude of the mitochondrial Ca²⁺ pool. A role for protein ADP-ribosylation in the intracellular regulation of mitochondrial Ca²⁺ homeostasis is suggested.

【 授权许可】

Unknown