Utrophin, the autosomal homologue of dystrophin, is widely‐expressed and membrane‐associated in cultured cell lines

FEBS Letters

Morris, G.E.¹  Thanh, LeThiet¹  Blake, D.J.²  thi Man, Nguyen¹  Davies, K.E.² 

[1] Research Division, N.E. Wales Institute, Deeside, Clwyd, CH5 4BR, UK;Molecular Genetics Group, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, OX3 9DU, UK

关键词: Dystrophin;    Dystrophin-related protein;    Muscular dystrophy;    DMDL gene;    Membrane-associated protein;   

DOI  :  10.1016/0014-5793(92)81174-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Utrophin, the autosomal dystrophin-related protein (DRP), is expressed in HeLa cells, smooth muscle-like BC₃HI cells from mouse brain, COS monkey kidney cells, the P38BD₁ monocyte-macrophage cell line and untransformed human skin fibroblasts, as well as in rat C6 glioma and Schwannoma cells. It was undetectable, however, in the Sp2/O mouse myeloma cell line and in hybridoma lines derived from it. Dystrophin was not detected in any of these cell lines. Although all utrophin-containing cells were capable of forming monolayers in culture, no major effects of either attachment to substratum or length of time in culture (2–17 days) on utrophin levels were observed. After subcellular fractionation ofBC₃HI or glioma cells, nearly all of the utrophin was found in the Triton-soluble fraction, suggesting an association with cell membranes.

【 授权许可】

Unknown