Cloning and sequencing of a rat type II activin receptor

FEBS Letters

Ito, Ikuro¹  Eto, Yuzuru²  Hasegawa, Yoshihisa¹  Minegishi, Takashi¹  Shinozaki, Hiromitsu¹  Miyamoto, Kaoru³  Ibuki, Yoshito¹  Nakamura, Manabu¹  Igarashi, Shigeo¹  Nakamura, Kazuto¹ 

[1] Department of Obstetrics and Gynecology, Gunma University School of Medicine, Maebashi, Gunma 371, Japan;Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki-ku, Kawasaki 210, Japan;National Cardiovascular Center, Research Institute, Fujishirodai, Suita, Osaka 565, Japan

关键词: Ovary;    Activin receptor;    mRNA;   

DOI  :  10.1016/0014-5793(92)81408-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length cDNA for a rat type II activin receptor was cloned by hybridization from a rat ovary cDNA library. The deduced amino acid sequence (513 residues) containing a single membrane-spanning domain and an intracellular kinase domain with predicted serine/threonine specificity. The amino acid sequence is 99.8% and 99.4% identical in the coding region with the previously cloned mouse and human type II activin receptor, and only 66.7% identical in the coding region with the previously cloned rat type IIB activin receptor. We examined the effect of PMSG-hCG on the mRNA level of type II activin receptor in immature rat ovaries. Northern blot analysis of ovarian RNA revealed two mRNAs (3.0 kb and 6.0 kb).

【 授权许可】

Unknown