Phenylalanyl‐tRNA synthetase from Thermus thermophilus can attach two molecules of phenylalanine to tRNAPhe

FEBS Letters

Lavrik, Ol'ga I.¹  Moor, Nina A.¹  Ankilova, Valentina N.¹  Stepanov, Victor G.¹ 

[1] Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Lavrentiev Prospect 8, 630090, Novosibirsk, Russia

关键词: Phenylalanyl-tRNA synthetase;    tRNAPhe;    tRNA aminoacylation;    PheRSase;    phenylalanyl-tRNA synthetase (EC 6.1.1.20);    hyper-Phe∼tRNA;    tRNA phenylalanylated above 100%;    Phe∼tRNA;    normally phenylalanylated tRNA;    RNase A;    ribonuclease A;   

DOI  :  10.1016/0014-5793(92)81099-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phenylalanyl-tRNA synthetase from the extreme thermophilic bacterium Thermus thermophilus can incorporate more than one molecule of phenylalanine into the tRNA^Phe. It is shown that the ‘hyperaminoncylated’ tRNAP^Phe is the bis-2′,3′-O-phenylalanyl-tRNA^Phe, and its formation is typical for the thermophilic enzyme but does not occur for E. coli phenylalanyl-tRNA synthetase under the same conditions.

【 授权许可】

Unknown