Saturation kinetics of coenzyme Q in NADH and succinate oxidation in beef heart mitochondria

FEBS Letters

Estornell, E.¹  Castelli, G.Parenti¹  Fato, R.¹  Castelluccio, C.¹  Cavazzoni, M.¹  Lenaz, G.¹ 

[1] Dipartimento di Biochimica, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy

关键词: Coenzyme;    Q;    NADH cytochrome c reductase;    Kinetics;    Beef heart mitochondrion;   

DOI  :  10.1016/0014-5793(92)81378-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The saturation kinetics of NADH and succinate oxidation for Coenzyme Q (CoQ) has been re-investigated in pentane-extracted lyophilized beef heart mitochondria reconstituted with exogenous CoQ₁₀. The apparent ‘K _m’ for CoQ₁₀ was one order of magnitude lower in succinate cytochrome c reductase than in NADH cytochrome c reductase. The K _m value in NADH oxidation approaches the natural CoQ content of beef heart mitochondria, whereas that in succinate oxidation is close to the content of respiratory chain enzymes.

【 授权许可】

Unknown