Identification of a small Na+/H+ exchanger‐like message in the rabbit myocardium

FEBS Letters

Dyck, Jason R.B.¹  Lopaschuk, Gary D.¹  Fliegel, Larry¹ 

[1] Department of Pediatrics, Faculty of Medicine, University of Alberta, Cardiovascular Disease Research Group, Edmonton, Alberta, Canada

关键词: Na+/H+ exchanger;    Myocardium;    mRNA;    Acidosis;    Ischemia;   

DOI  :  10.1016/0014-5793(92)81343-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We examined the Na⁺/H⁺ exchanger message in isolated perfused rabbit hearts using Northern blot analysis with cDNA encoding for the rabbit cardiac Na⁺/H⁺ exchanger. A cDNA probe from the coding region of the rabbit myocardial Na⁺/H⁺ exchanger hybridized to mRNA of 5 kb under high stringency, and to a second 3.8 kb mRNA species under low stringency. When Northern blots were re-probed with a section of the 3′-untranslated region of the cDNA, the 5 kb message was apparent while the smaller 3.8 kb message was not. If isolated working rabbit hearts were subjected to ischemia we observed increases in the 3.8 kb message. Overall, the results show that a 3.8 kb mRNA product, which is homologous to the amiloride sensitive Na⁺/H⁺ exchanger, exists in the myocardium and increases during ischemia in the myocardium.

【 授权许可】

Unknown