Human Interleukin‐1 receptor antagonist High yield expression in E. coli and examination of cysteine residues

FEBS Letters

Solari, Roberto³  Mott, Helen R.²  Sim, Robert B.⁴  Steinkasserer, Alexander⁴  Willis, Anthony C.⁴  Aplin, Robin T.¹  Robinson, Carol C.¹ 

[1] Dyson Perrins Laboratories, University of Oxford, South Parks Road, Oxford, OX1 3QY, UK;Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK;Yamanouchi Research Institute (UK), Littlemore Hospital, Oxford, OX4 4XN, UK;MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

关键词: IL-1 receptor antagonist;    E. coli expression;    Cysteine mapping;   

DOI  :  10.1016/0014-5793(92)81147-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification, This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE₂ production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked.

【 授权许可】

Unknown