期刊论文详细信息
FEBS Letters
Human Interleukin‐1 receptor antagonist High yield expression in E. coli and examination of cysteine residues
Solari, Roberto3  Mott, Helen R.2  Sim, Robert B.4  Steinkasserer, Alexander4  Willis, Anthony C.4  Aplin, Robin T.1  Robinson, Carol C.1 
[1] Dyson Perrins Laboratories, University of Oxford, South Parks Road, Oxford, OX1 3QY, UK;Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK;Yamanouchi Research Institute (UK), Littlemore Hospital, Oxford, OX4 4XN, UK;MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
关键词: IL-1 receptor antagonist;    E. coli expression;    Cysteine mapping;   
DOI  :  10.1016/0014-5793(92)81147-E
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification, This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked.

【 授权许可】

Unknown   

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