Molecular cloning and functional expression of the second mouse nm23/NDP kinase gene, nm23‐M2

FEBS Letters

Urano, Takeshi¹  Takamiya, Kogo¹  Furukawa, Koichi¹  Shiku, Hiroshi¹ 

[1] Department of Oncology, Nagasaki University School of Medicine, 12-4, Sakamoto-machi, Nagasaki 852, Japan

关键词: nm23/nucleoxide diphosphate kinase;    Metastatic suppressor gene;    nm23-M2 gene;    Monoclonal antibody;    NDP kinase;    nucleotide diphosphate kinase;    mAb;    monoclonal antibody;    RT;    reverse transcription;    PCR;    polymerase chain reaction;    GST;    glutathione S-transferase;   

DOI  :  10.1016/0014-5793(92)80807-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new murine cDNA of nm23/NDP kinase was isolated. A RT-PCR product was obtained from the normal mouse liver mRNA with primers designed for the human nm23-H2 gene. The product was used as a probe to screen a cDNA library from the murine melanoma cell line. B16, and two clones containing the entire open reading frame were obtained. It was predicted that the DNA sequence encoded 152 amino acids which was 98% identical to the nm23-H2 protein. The entire nm23-M1 and -M2 gene-coding regions were translated as fusion proteins with a glutathione S-transferase. These fusion proteins displayed NDP kinase activities.

【 授权许可】

Unknown