Vanadate potentiates the glycogenic action of insulin‐like growth factors on isolated diaphragm

FEBS Letters

Bouillon, Roger²  Bollen, Mathieu¹  Stalmans, Willy¹  Vandorpe, Greet¹  Van Herck, Erik² 

[1] Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Belgium;Laboratorium voor Experimentele Geneeskunde en Endocrinologie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, Belgium

关键词: Vanadate;    Insulin-like growth factor I;    Insulin-like growth factor II;    Insulin;    Glycogen;    Diaphragm;   

DOI  :  10.1016/0014-5793(92)81096-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Na₃VO₄ (6.5 μmol/100 g rat weight), co-injected with a trace amount of [^???C]glucose, increased within 15 min the incorporation of radiolabel in diaphragmal glycogen. After 2 h the vanadate-induced increases were 12-fold in the diaphragm and 7–8-fold in heart and liver. In contrast, when added to isolated diaphragms for up to 1 h, vanadate (0.1–5 mM) had no effect on the synthesis of glycogen from 5 mM glucose. In search of a putative mediator of vanadate's action in vivo, insulin and the insulin-like growth factors (IGFs) were considered. Their plasma concentration was not affected by vanadate treatment. In isolated diaphragms, 1 mM vanadate did not potentiate insulin-induced glycogen synthesis, but it caused a several-fold increase in glycogen synthesis in the presence of concentrations of IGF-I which, alone, had no effect. A similar synergism occurred between vanadate and IGF-II. We propose that the glycogenic action of vanadate in vivo, at least in some tissues, involves a potentiation of the action of IGF-I.

【 授权许可】

Unknown