Reactive oxygen species are involved in the activation of cellular phospholipase A2

FEBS Letters

Goldman, R.³  Ferber, E.¹  Zort, U.² 

[1] Max-Planck-Institut für Immunbiologie, Freiburg, Germany;Department of Hormone Research, The Weizmann Institute of Science, Rehovot, Israel;Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel

关键词: Phospholipase A2;    NADPH oxidase;    Reactive oxygen species;    Diphenylene iodonium;    Vanadate;    Tyrosine Protein phosphatase;    V;    vanadate;    H;    H2O2;    PKC;    protein kinase C;    PLA2;    phospholipase A2;    TPPase;    tyrosine Protein phosphatase;    CL;    chemiluminescence;    DPI;    diphenylene iodonium;    ROS;    reactive oxygen species;    A;    Ca2+ ionophore A23187;    AA;    arachidonic acid;    T;    12-O-tetradecanoyl phorbol 13-acetate;    OZ;    opsonized zymosan;    St;    staurosporine;    Herb;    A;    herbimycin A;    BMDM;    bone marrow derived macrophages;    Tg-Mo;    thioglycollate-elicited peritoneal macrophages;   

DOI  :  10.1016/0014-5793(92)81092-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vanadate (V) potentiated (4- to 10-fold) the activation of cellular phospholipase A₂ (PLA₂) induced by H2O2 (H), a phorbol ester (T), a Ca²⁺-ionophore (A) and opsonized zymosan in macrophages. V+H induced in intact cells the activation and translocation of PLA₂ and protein kinase C(PKC) to the plasma membrane. V+H and V+T+A induced strong chemiluminescence (CL) which was abrogated by a specific NADPH oxidase inhibitor diphenylene iodonium (DPI). DPI markedly suppressed the stimulation of PLA₂ by V+T+A and V+OZ. The results suggest that the formation of endogenous reactive oxygen species (ROS) is important for PLA₂ activation.

【 授权许可】

Unknown