期刊论文详细信息
FEBS Letters | |
Interaction of plasma gelsolin with tropomyosin | |
Koepf, Edward K.1  Burtnick, Leslie D.1  | |
[1] Department of Chemistry, University of British Columbia, Vancouver, BC V6T, 1Z1, Canada | |
关键词: Gelsolin; Tropomyosin; TM; tropomyosin; FITC; fluorescein-5-isothiocyanate; BITC; benzophenone-4-isothiocyanate; | |
DOI : 10.1016/0014-5793(92)80738-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labelled gelsolin on tropomyosin-agarose affinity columns and by the ability of tropomyosin to cause an increase in the fluorescence intensity of gelsolin labelled with fluorescein-5-isothiocyanate. Both of these effects require the presence of calcium ions.
【 授权许可】
Unknown
【 预 览 】
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