期刊论文详细信息
FEBS Letters
Interaction of plasma gelsolin with tropomyosin
Koepf, Edward K.1  Burtnick, Leslie D.1 
[1] Department of Chemistry, University of British Columbia, Vancouver, BC V6T, 1Z1, Canada
关键词: Gelsolin;    Tropomyosin;    TM;    tropomyosin;    FITC;    fluorescein-5-isothiocyanate;    BITC;    benzophenone-4-isothiocyanate;   
DOI  :  10.1016/0014-5793(92)80738-3
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labelled gelsolin on tropomyosin-agarose affinity columns and by the ability of tropomyosin to cause an increase in the fluorescence intensity of gelsolin labelled with fluorescein-5-isothiocyanate. Both of these effects require the presence of calcium ions.

【 授权许可】

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