Glycopeptide of P0 protein inhibits homophilic cell adhesion Competition assay with transformants and peptides

FEBS Letters

Toya, Shigeo¹  Miura, Masayuki³  Uyemura, Keiichi³  Asou, Hiroaki³  Yazaki, Takahito³  Kitamura, Kunio² 

[1]Departments of Neurosurgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

[2]Department of Physiology, Saitama Medical School, Saitama 350-04, Japan

[3]Departments of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan

关键词: P0;    Homophilic adhesion;    Immunoglobulin superfamily;    Glycopeptide;    Myelin;   

DOI  :  10.1016/0014-5793(92)80713-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of major myelin glycoprotein P0 by PO cDNA transfection into C6 glioma cells promoted homophilic cell adhesion of the cells. After the dissociated cells were incubated for various times, the number of particles at each time point was measured. The total number of particles decreased to 24% in 60 min for transformant (C6P0) cells, in contrast to only 68% for control (C6P0′) cells. To confirm the homophilic mechanism of adhesion, mixed-cell aggregation experiments were performed. Among the four synthetic peptides corresponding to a part of the P0 sequence used, only peptide 3 (residues 90–96), which contained a carbohydrate attaching site, caused considerable inhibition of cell aggregation (approximately 50%). In addition, the glycopeptide (residues 91–95) obtained from bovine P0 markedly inhibited cell aggregation (by approximately 85%).

【 授权许可】

Unknown