Stoichiometric binding of 2′(or 3′)‐O‐(2,4,6‐trinitrophenyl)adenosine 5′‐triphosphate to bovine heart cytochrome c oxidase

FEBS Letters

Kadenbach, Bernhard¹  Reimann, Achim¹ 

[1] Fachbereich Chemie, der Philipps-Universität, Hans Meerwein Straße, D-3550 Marburg, Germany

关键词: Cytochrome c oxidase;    TNP-ATP;    Nucleotide binding;    Allosteric effector;   

DOI  :  10.1016/0014-5793(92)80698-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of 2′(or 3′)-O-(2,4,6-trinitrophenyl)adenosine 5′-triphosphate (TNP-ATP) to isolated bovine heart cytochrome c oxidase (COX) was studied by following its specific spectral change at 510 nm. The quantitative titration revealed two binding sites for TNP-ATP per monomer COX with a K _d of 1.6 μM.

【 授权许可】

Unknown