Direct transfer of molybdopterin cofactor to aponitrate reductase from a carrier protein in Chlamydomonas reinhardtii

FEBS Letters

Aguilar, Miguel¹  Fernández, Emilio¹  Kalakoutskii, Kyrill¹  Cárdenas, Jacobo¹ 

[1] Departamento de Bioquímica y Biologia Molecular, Facultad de Ciencias, Univesidad de Córdoba, 14071-Córdoba, Spain

关键词: Molybdenum cofactor;    Molybdopterin;    Nitrite reductase;    Xanthine oxidase;    CP;    carrier protein;    MoCo;    molybdenum cofactor;    NR;    nitrate reductase;    XO;    xanthine oxidase;   

DOI  :  10.1016/0014-5793(92)80758-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Chlamydomonas reinhardtii molybdenum cofactor (MoCo)-carrier protein (CP), capable of reconstituting nitrate reductase activity with apoprotein from the Neurospora crassa mutant nit-1, was subjected to experiments of diffusion through a dialysis membrane and gel filtration. CP bonded firmly MoCo and did not release it efficiently unless aponitrate reductase was present in the incubation mixture, Stability of MoCo bound to CP against air and heat was very similar to that of free-MoCo released from milk xanthine oxidase. Our data strongly suggest that MoCo is directly transferred from CP to aponitrate reductase to form an active enzyme.

【 授权许可】

Unknown