Crossing three membranes Channel formation by aerolysin

FEBS Letters

Buckley, J.Thomas¹ 

[1] Department of Biochemistry and Microbiology, University of Victoria, Box 3055, Victoria, B.C., V8W 3P6, Canada

关键词: Aerolysin;    Hemolysin;    Proteolytic activation;    Channel formation;   

DOI  :  10.1016/0014-5793(92)80896-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Aerolysin is a channel-forming toxin responsible for the pathogenicity of Aeromonas hydrophila. It crosses the inner and outer membranes of the bacteria in separate steps and is released as a 52-kDa inactive protoxin which is activated by proteolytic removal of approximately 40 amino acids from the C terminus. The toxin binds to the erythrocyte transmembrane protein glycophorin and oligomerizes before inserting into the membrane, producing a voltage gated, anion selective channel about 1 nm in diameter. Remarkably, proaerolysin appears to be dimeric, whereas the oligomeris a heptamer. Using chemical modification and site-directed mutagenesis, we have identified some of the regions of the molecule which appear to be involved in secretion and in channel formation.

【 授权许可】

Unknown