Barley (1→3,1→4)‐β‐glucanase isoenzyme EI gene expression is mediated by auxin and gibberellic acid

FEBS Letters

Slakeski, Nada¹  Fincher, Geoffrey B.¹ 

[1] Department of Biochemistry, La Trobe University, Bundoora, Victoria 3083, Australia

关键词: Auxin;    Barley;    Gibberellic acid;    (1→3;    1→4)-β-Glucanase;    Tissue-specific expression;   

DOI  :  10.1016/0014-5793(92)80977-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of young barley leaves with indole acetic acid (IAA) or gibberellic acid (GA₃) results in a dramatic increase in levels of (1→3,1→4)-β-glucanase isoenzyme El transcripts. In young roots of comparable age, levels of isoenzyme El mRNA are high; IAA inhibits expression while GA₃ has no effect on mRNA levels. The addition of both abscisic acid and GA₃ to leaves, roots and aleurone layers leads to higher levels of (1→3,1→4)-β-glucanase isoenzyme El mRNA than is found with Ga₃ alone. Little or no expression of (1→3,1→4)-β-glucanase isoenzyme EII is detected in vegetative tissues, but in isolated aleurone layers GA₃ enhances levels of isoenzyme EII transcripts, as does IAA. Thus, the two barley (1→3,1→4)-β-glucanase genes respond quite differently to phytohormone treatment, depending on the tissue and its stage of development.

【 授权许可】

Unknown