Solubilization steps of dark‐adapted purple membrane by Triton X‐100 A spectroscopic study

FEBS Letters

Paternostre, Marie-Thérèse¹  Meyer, Olivier¹  Ollivon, Michel¹ 

[1]Equipe Physico-Chimie des Systèmes Polyphasès, CNRS URA 1218, Université Paris-Sud, 5 rue J.B Clément, 92296 Chátenay-Malabry Cedex, France

关键词: Purple membrane;    Bacteriorhodopsin;    Triton X-100;    Solubilization;    Turbidity;    Absorbance;    PM;    purple membrane;    BR;    bacteriorhodopsin;    TX100;    Triton X-100;    LIP;    lipid;    OD;    optical density;    Rcπ;    effective TX100 to PM components ratio at break points;    HEPES;    N(2-hydroxyethyl)-piperazine-N′-2-ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(92)80679-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ultraviolet-visible spectroscopy has been used to follow the solubilization of the dark-adapted purple membrane of Halobacterium halobium by Triton X-100. Turbidity of purple membrane fragments and absorbance of bacteriorhodopsin variations during continuous addition of detergent give solubilization profiles exhibiting several break points corresponding to different equilibrium stages of the solubilization process. The present method allows the determination of the detergent to protein + lipid ratio in mixed aggregates at the corresponding break points. It was concluded that, when performed systematically, this technique is a very convenient and powerful tool for the quantitative study of biomembrane-to-micelle transition.

【 授权许可】

Unknown