Interaction between oleic acid‐containing pH‐sensitive and plain liposomes Fluorescent spectroscopy studies

FEBS Letters

Lukyanov, A.N.³  Torchilin, V.P.¹  Klibanov, A.L.⁴  Omelyanenko, V.G.² 

[1]Center for Imaging and Pharmaceutical Research, Massachusetts General Hospital-East, Charlestown, MA 02129, USA

[2]Department of Pharmaceutics, University of Utah, Salt Lake City, UT 84108, USA

[3]Institute of Experimental Cardiology, USSR Cardiology Research Center, Moscow 121552, USSR

[4]Department of Pharmacology, University of Pittsburg, PA 15261, USA

关键词: pH-sensitive liposome;    Membrane fusion;    Oleic acid;    Energy transfer method;    PE;    egg phosphatidyl ethanolamine;    PC;    egg phosphatidyl choline;    Chol;    cholesterol;    OA;    oleic acid;    SRh;    sulforhodamine B;    [14C]PE;    [14C]dioleoyl PE;    Rh-PE;    N-(lysamine rhodamine B sulfamine)-PE;    NBD-PE;    N-(7-nitro-1;    2;    3-benzoxadiazol-4-yl)-PE;    pH-Lip;    pH-sensitive liposomes;    P-Lip;    plain liposomes;    FT;    liposomes;    prepared by freeze-thawing method;    REV;    liposomes prepared by reverse phase evaporation;    PBS;    phosphate-buffered saline;    TLC;    thin layer chromatography;   

DOI  :  10.1016/0014-5793(92)80663-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The energy transfer method has been applied to study the interaction between pH-sensitive liposomes (phosphatidyl ethanolamine/oleic acid/cholesterol, 4:2:4 molar ratio) and plain liposomes (phosphatidyl choline/phosphatidyl ethanolamine/cholesterol, 4:2:3 molar ratio). It was shown that a slow fusion process occurs between two types of liposomes. Also, the transfer of oleic acid from pH-sensitive liposomes to plain liposomes takes place. This transfer results in the increased permeability of both pH-sensitive and plain liposomes, facilitating the release of liposome-entrapped fluorescent dye. The data obtained were used for a possible explanation of the mechanism of intracytoplasmic drug delivery by pH-sensitive oleic acid-containing liposomes.

【 授权许可】

Unknown