Detection of a near infra‐red absorption band of ferrohaem a3in cytochrome c oxidase

FEBS Letters

Ingledew, W.John²  Rich, Peter R.¹  Moody, A.John¹ 

[1] Glynn Research Institute, Bodmin, Cornwall, PL30 4AU, UK;Department of Biochemistry and Microbiology, University of St. Andrews, St. Andrews, Fife, KY16 9AL, Scottland, UK

关键词: Cytochrome c oxidase;    Carbon monoxide;    Cyanide;    Haem a3;    Spectroscopy;   

DOI  :  10.1016/0014-5793(92)80659-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have detected weak absorption bands in the near infra-red region of reduced mammalian cytochrome c oxidase, analogous to those that we have recently reported to be present in the bacterial cytochrome o (Ingledew, W.J., Bacon, M. and Rich, P.R. (1992) FEBS Lett. 305, 167–170). The major band is centred at 784 nm and has an εmM¹cm¹ of around 0.1. It is shifted to 760 nm in the carbon monoxide compound and is absent in the reduced cyanide complex. We attribute it to a charge transfer band of ferrohaem a₃ , equivalent to the ‘band III’ or ‘conformational band’ of haemoglobin.

【 授权许可】

Unknown