FEBS Letters | |
Stimulation of human nitric oxide synthase by tetrahydrobiopterin and selective binding of the cofactor | |
Werner, Ernst R.3  Klatt, Peter2  Werner-Felmayer, Gabriele3  Ambach, Edda1  Wachter, Helmut3  Mayer, Bernd2  Heinzel, Burghard2  | |
[1] Institut für Gerichtliche Medizin der Universität Innsbruck, Austria;Institut für Pharmakologie der Freien Universität Berlin, Germany;Institut für Medizinische Chemie und Biochemie der Universität Innsbruck, Austria | |
关键词: Nitric oxide synthase; Human cerebellum; Neopterin; Tetrahydrobiopterin; NO; nitric oxide; HPLC; high performance liquid chromatography; PAGE; polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(92)80886-L | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To check the stimulatory potency of the tetrahydro forms of the two major pteridines occurring in human tissues, neopterin and biopterin, NO synthase was purified 6000-fold from human cerebellum. Tetrahydrobiopterin stimulated the activity up to 4.5-fold in a concentration dependent manner with a maximum above 1 μM, whereas tetrahydroneopterin was completely inactive in concentrations up to 100 μM. Tetrahydrobiopterin, but not neopterin derivatives, were copurified with the NO synthase activity. Our results demonstrate that human cerebellum contains a tetrahydrobiopterin dependent NO synthase activity.
【 授权许可】
Unknown
【 预 览 】
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