Stimulation of human nitric oxide synthase by tetrahydrobiopterin and selective binding of the cofactor

FEBS Letters

Werner, Ernst R.³  Klatt, Peter²  Werner-Felmayer, Gabriele³  Ambach, Edda¹  Wachter, Helmut³  Mayer, Bernd²  Heinzel, Burghard² 

[1] Institut für Gerichtliche Medizin der Universität Innsbruck, Austria;Institut für Pharmakologie der Freien Universität Berlin, Germany;Institut für Medizinische Chemie und Biochemie der Universität Innsbruck, Austria

关键词: Nitric oxide synthase;    Human cerebellum;    Neopterin;    Tetrahydrobiopterin;    NO;    nitric oxide;    HPLC;    high performance liquid chromatography;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(92)80886-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To check the stimulatory potency of the tetrahydro forms of the two major pteridines occurring in human tissues, neopterin and biopterin, NO synthase was purified 6000-fold from human cerebellum. Tetrahydrobiopterin stimulated the activity up to 4.5-fold in a concentration dependent manner with a maximum above 1 μM, whereas tetrahydroneopterin was completely inactive in concentrations up to 100 μM. Tetrahydrobiopterin, but not neopterin derivatives, were copurified with the NO synthase activity. Our results demonstrate that human cerebellum contains a tetrahydrobiopterin dependent NO synthase activity.

【 授权许可】

Unknown