Heat shock proteins of barley mitochondria and chloroplasts Identification of organellar hsp 10 and 12: putative chaperonin 10 homologues

FEBS Letters

Hartman, Dallas J.¹  Dougan, David¹  Høj, Peter B.¹  Hoogenraad, Nicholas J.¹ 

[1] Department of Biochemistry, La Trobe University, Bundoora, Victoria, 3083 Australia

关键词: Heat shock protein;    Chaperonin;    Chloroplast;    Mitochondrion;    Barley;    Rat;    Metabolic labelling;    Electrophoresis;    cpn60;    chaperonin 60;    cpn10;    chaperonin 10;    hsp;    heat shock protein;    DMEM;    Dulbecco's modified Eagle's medium;    MW;    molecular weight;   

DOI  :  10.1016/0014-5793(92)80883-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tissue slices from barley seedlings were subjected to heat shock and metabolically labelled with [³⁵S]methionine and [³⁵S]lcysteine, Mitochondria and chloroplasts were isolated and shown to contain two novel heat shock proteins of 10 and 12 kDa, respectively. The possibility that these proteins, like a mitochondrial 10 kDa stress protein recently isolated from rat hepatoma cells [(1992) Proc. Natl. Acad. Sci. 89, in press] represent eukaryotic chaperonin 10 homologues is discussed.

【 授权许可】

Unknown