Synthesis and characterization of a 29‐amino acid residue DNA‐binding peptide derived from α/β‐type small, acid‐soluble spore proteins (SASP) of bacteria

FEBS Letters

Setlow, Peter¹  Mohr, Scott C.²  Rao, Hai²  Fairhead, Heather¹ 

[1] Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030-3305, USA;Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, MA 02215, USA

关键词: DNA-binding peptide;    SASP;    Circular dichroism;    DNase protection;    DNA photochemistry;    SASP;    small;    acid-soluble spore protein(s);    CD;    circular dichroism;    Fmoc-;    N-(9-fluorenylmethoxycarbonyl)-;    PepSyn KA;    polyacrylamide kieselguhr modified for amino acid attachment - Trademark of Milligen/Biosearch;    Inc;    TFA;    trifluoroacetic acid;    TFE;    2;    2;    2-trifluoroethanol;   

DOI  :  10.1016/0014-5793(92)80876-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 29-amino acid residue peptide (SASP-peptide) derived from the sequence of the putative DNA-contacting portion at the carboxyl terminus of an α/β-type small, acid-soluble spore protein (SASP) of Bacillus subillis has been synthesized by automated solid-phase methods and tested for its ability to interact with DNA. Circular dichroism (CD) spectroscopy reveals an interaction between this SASP-peptidc and DNA, both by an increase in α-helix content of the peplide (which alone has a mostly random conformation) and by enhancement of the 275-nm CD band of the DNA. In contrast to results with intact α/β-type SASP, however, the peptide does not induce a B → A cenformational transition in the DNA. The SASP-peptide also binds to poly(dG)·poly(dC) and prtects this polynucleotide against DNase I digestion and UV light-induced cylosine dimer formation, parallel to Findings made previously with native α/β-type SASP. The results confirm the hypothesis that the carboxyl-terminal region of the α/β-type SASP directly contacts DNA and possesses some, but not all, or the functional characteristics of the intact molecule.

【 授权许可】

Unknown