Extracellular 2‐chloroadenosine and ATP stimulate volume‐sensitive Cl− current and calcium mobilization in human tracheal 9HTEo− cells

FEBS Letters

Gruenert, Dieter C.²  Zottini, Michela¹  Rugolo, Michela¹  Mastrocola, Teresa¹  Galietta, Luis J.V.³  Romeo, Giovanni³  Rasola, Andrea³ 

[1] Dipartimento di Biologia E.S., Università di Bologna, v. Irnerio 42, 40126, Bologna, Italy;Department of Laboratory Medicine, Cardiovascular and Cancer Research Institute, University of California, San Francisco, CA 94143, USA;Laboratorio di Genetica Molecolare, Ist. G. Gaslini, 16148 Genova, Italy

关键词: Airway epithelium;    Cl− current;    Cell volume;    2-Chloroadenosine;    ATP;    Intracellular Ca2+;    2-CADO;    2-chloroadenosine;    DMPX;    3;    7-dimethyl-1-propargylxanthine;    EGTA;    ethyleneglycol-bis-(β-aminoethyl)-N;    N;    N′;    N′-tetraacetic acid;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(92)80589-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The perforated-patch whole-cell technique was used to record membrane currents in epithelial cells (9HTEo−) obtained from the human tracheal epithelium. Extracellular application of 2-chloroadenosine and ATP (0.01–100 μM) caused activation of Cl⁻ currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca²⁺ or by the addition of the A₂ adenosine receptor antagonist 3,7-dimethyl-1-propargylxanthine (DMPX). Fluorimetric measurements with fura-2 reveal that 2-chloroadenosine and ATP elicited both a Ca²⁺ influx through the plasma membrane and a release from intracellular stores.

【 授权许可】

Unknown