C‐CAM (Cell‐CAM 105) is a calmodulin binding protein

FEBS Letters

Wikström, Tarja²  Öbrink, Björn¹  Blikstad, Ingrid B.²  Aurivillius, Magnus¹ 

[1] Department of Medical Cell Biology, Medical Nobel Institute, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden;Department of Medical and Physiological Chemistry, University of Uppsala, The Biochemical Center, Box 575, S-751 23 Uppsala, Sweden

关键词: Cell adhesion molecule (CAM);    Calmodulin;    Cell adhesion;    Transmembrane signalling;   

DOI  :  10.1016/0014-5793(92)80276-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

C-CAM (Cell-CAM 105) is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily. It mediates intercellular adhesion of rat hepatocytes and occurs in various isoforms in several epithelia, vessel endothelia and leukocytes. We now report that purified liver C-CAM interacts specifically with calmodulin. Binding was observed both when ¹²⁵I-labeled C-CAM was used in a dot-blot assay and when ¹²⁵I-labeled calmodulin was used in a gel overlay assay. Experiments with protease-generated peptides indicated that calmodulin bound to the cytoplasmic domain of C-CAM. Analyses of whole liver membranes demonstrated that C-CAM is one of five major proteins that bind calimodulin in a calcium-dependent manner.

【 授权许可】

Unknown