Ornithine decarboxylase from Crithidia fasciculata is metabolically unstable and resistant to polyamine down‐regulation

FEBS Letters

Ceriani, Carolina¹  Algranati, Israel D.¹  González, Nélida S.¹ 

[1] Instituto de Investigaciones Bioquímicas ‘Fundación Campomar’, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and CONICET, A. Machado 151, 1405 Buenos Aires, Argentina

关键词: Ornithine decarboxylase;    Enzyme turnover;    Polyamine-dependent regulation;    Crithidia fasciculata;    ODC;    ornithine decarboxylase;    DFMO;    α-di-fluoromethylornithine;   

DOI  :  10.1016/0014-5793(92)80253-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ornithine decarboxylase (ODC) of Crithidia fasciculata extracts shows maximal activity during exponential growth of the parasite and decreases markedly in the stationary phase. The inhibition of protein synthesis by cycloheximide evoked a rapid loss of enzyme activity with a half-life of about 30 min. Upon removal of DFMO from Crithidia cultures treated with the drug for 24 h, the ODC activity increased at the same rate as total protein synthesis. The addition of putrescine at high concentrations to parasites cultivated in a synthetic medium showed that Crithidia CDC levels were not reduced by polyamines.

【 授权许可】

Unknown