The consensus sequences for cdc2 kinase and for casein kinase‐2 are mutually incompatible A study with peptides derived from the β‐subunit of casein kinase‐2

FEBS Letters

Pinna, Lorenzo A.¹  Marin, Oriano¹  Draetta, Giulio²  Meggio, Flavio¹ 

[1] Dipartimento di Biologica and Centro per lo Studio della Fisiologia Mitocondriale del Consiglio Nazionale delle Ricerche, Università di Padova, Padova, Italy;European Molecular Biology Laboratory, Heidelberg, Germany

关键词: Casein kinase-2;    cdc2;    Protein phosphorylation;    Site specificity;    Consensus sequence;    CK2;    casein kinase-2;    cdc2;    cell division cycle protein kinase;    Sp;    phosphoserine;    Tp;    phosphothreonine;    Yp;    phosphotyrosine;   

DOI  :  10.1016/0014-5793(92)80221-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two series of synthetic peptides that reproduce the amino- and carboxyl-terminal segments of the β-subunit of casein kinase-2, including the sites phosphorylated by CK2 and cdc2 kinase, respectively, have been used as model substrates for these enzymes. The N-terminal peptide β(1–9), MSSSEEVSW, is readily phosphorylated by CK2 but not at all by cdc2. The opposite is true of the C-terminal peptide β(206–215), NFKSPVKTIR. whose Ser-4 is a good target for cdc2 while being unaffected by CK2. The individual substitutions of Pro-5 and Lys-7 in the latter peptide with Gly and Ala (or Glu), respectively, prevent its phosphorylation by cdc2, whereas the substitution of Lys-3 with Ala is well tolerated and the substitution of the target Ser with Thr actually improves phosphorylation. Thus the consensus sequence for cdc2 is shown to be X-S-P-X-K. Such a requirement of a basic residue at Position +3 is opposite to that of CK2 whose consensus sequence (S-X-X-E/D/Yp/Sp) includes an acidic residue at the same position. Moreover the motif Ser-Pro is detrimental for CK2, preventing the phosphorylation of otherwise suitable peptides. These observations would rule out the possibility that the site specificity of CK2 might overlap with that of cdc2 and possibly of other Pro-directed protein kinases.

【 授权许可】

Unknown