A consensus repeat sequence from the human insulin gene linked polymorphic region adopts multiple quadriplex DNA structures in vitro

FEBS Letters

Hammond-Kosack, Michael C.U.¹  Docherty, Keyin¹ 

[1] Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, Birmingham B15 2TH, UK

关键词: G4 DNA;    Insulin gene linked polymorphic region;    Insulin gene;    DNA structure;    ILPR;    insulin gene linked polymorphic region;    BAA;    bromoacetaldehyde;   

DOI  :  10.1016/0014-5793(92)80214-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A hypervariable region consisting of repeats of a 14 base pair (bp) consensus sequence ACAGGGGT(G/C)(T/C)GGGG is located 363 bp upstream of the human insulin gene. Different repeat numbers if this oligonucleotide give rise to a polymorphism, and so this region is commonly known as the insulin gene linked polymorphic region (ILPR). Here we present evidence, based on the mobility in non-denaturing polyacrylamide gels of two dissimilarly sized oligonucleotides containing the ILPR consensus sequence, that this sequence can adopt a number of quadriplex DNA structures in vitro.

【 授权许可】

Unknown