Cloning and characterization of the rat glutathione peroxidase gene

FEBS Letters

Ho, Ye-Shih¹  Howard, Adriann J.¹ 

[1] Laboratory of Molecular Biology, Division of Allergy, Critical Care and Respiratory Medicine, Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA

关键词: Genomic cloning;    S1 nuclease mapping;    Primer extension;    Regulatory sequence;   

DOI  :  10.1016/0014-5793(92)80198-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The increased activity of glutathione peroxidase (GSHPx) in rat lungs is associated with the development of tolerance of the animals to hyperoxia. To understand further the regulation of expression of this enzyme, the molecular structure of the corresponding rat gene was characterized. The rat GSHPx gene consists of two exons interrupted by a single intron of 217 base pairs. The same initiation sites for transcription were found to be utilized in both lung and liver. The promoter of the GSHPx gene contains neither a ‘TATA’ box nor a ‘CAAT’ box. Instead, it comprises two copies of Sp1 binding motif and one copy of AP-2 binding motif. These features of the promoter may offer a clue to the mechanisms by which the expression of this gene is controlled.

【 授权许可】

Unknown