Differential sensitivity of CG and CCG DNA sequences to ethionine‐induced hypomethylation of the Nicotiana tabacum genome

FEBS Letters

Bezdĕk, Milan¹  Kuhrová, Viera¹  Koukalová, Blaz̆ena¹  Vyskot, Boris¹ 

[1] Insitute of Biophysics, Czechoslovak Academy of Sciences, 612 65 Brno, Czechoslovakia

关键词: Plant DNA;    CG and CCG DNA sequence;    Ethionine-induced hypomethylation;    Nicotiana tabacum;    5mC;    5-methylcytosine;    Ethi;    ethionine;    5-azaC;    5-azacytidine;    MTase;    DNA methyltransferase;    SAM;    S-adenosyl-L-methionine;    MS medium;    Murashige-Skoog's medium;    CTBA;    cetyltrimethylammoniumbromide;    Dam;    DNA adenine methyltransferase;   

DOI  :  10.1016/0014-5793(92)80860-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Plant DNA is distinguished from the DNA of all other organisms by its high content of 5-methylcytosine (5mC). 5mC levels may amount to 30% of total cytosines, distributed between the sequences CG and CXG. The results presented here show that the methylation status of CXG sequences could be influenced by culturing tobacco tissues on subtoxic concentrations of ethionine. The hypomethylating effect of ethionine, evaluated as the capability of MspI or HpaII to cleave the DNA, proved to be rather specific for CCG and differed from that or 5-azacytidine which did not discriminate between CG and CXG sequences.

【 授权许可】

Unknown