Escherichia coli leucine‐responsive regulatory protein (Lrp) controls lysyl‐tRNA synthetase expression

FEBS Letters

Delort, Florence¹  Blanquet, Sylvain¹  Plateau, Pierre¹  Dessen, Philippe¹  Gazeau, Myriam¹ 

[1] Laboratoire de Biochimie, Unité de Recherche Associée 240 du Centre National de la Recherche Scientifique, Ecole Polytechnique, 91128 Palaiseau Cedex, France

关键词: Lysyl-tRNA synthetase;    Leucine-responsive regulatory protein;    lysU;    lrp;    Leucine;    Escherichia coli;   

DOI  :  10.1016/0014-5793(92)80857-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using random Tn10 insertion mutagenesis, we isolated an Escherichia coli mutant strain affected in the regulation of lysU, the gene encoding the inducible form of lysyl-tRNA synthetase. The transposon giving rise to the altered expression of lysU was found inserted within lrp. The latter gene codes for the leucine-responsive regulatory protein (Lrp) which mediates a global response of the bacterium to leucine. An involvement of Lrp in the regulation of lysU was searched for by using a lysU-lacZ operon fusion. The following conclusions were reached: (i) inactivation of lrp causes an increased activity of the lysU promoter, whatever the growth conditions assayed, (ii) insertion of a wild-type lrp gene into a multi-copy plasmid significantly reduces lysU expression, and (iii) sensitivity of the lysU promoter to the presence of leucine in the growth medium is abolished in the lrp context.

【 授权许可】

Unknown