Inhibition of neutrophil superoxide production by human plasma α1‐antitrypsin

FEBS Letters

Blake, David R.¹  Winyard, Paul G.¹  Chidwick, Keith¹  Bucurenci, Nadia¹ 

[1] Inflammation Research Group, Arthritis and Rheumatism Council Building, London Hospital Medical College, 25-29 Ashfield Street, London E1 2AD, England

关键词: α1-Antitrypsin;    Serine protease inhibitor;    Neutrophil respiratory burst;    Superoxide anion radical;    Inflammation;    Human;    α1-AT;    α1-antitrypsin;    TPCK;    l-I-tosylamide 2-phenylethyl chloromethyl ketone;    SBTI;    soybean trypsin inhibitor;    Con A;    concanavalin A;    Cyto E;    cytochalasin E;   

DOI  :  10.1016/0014-5793(92)80156-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report here that human plasma α₁-antitrypsin (α₁-AT) inhibited human neutrophil O₂ ⁻ release elicited by a variety of stimulants. In comparison, the inhibitory capacities of two serine protease inhibitors, l-1-tosylamide 2-phenylethylchloromethyl ketone (TPCK) and soybean trypsin inhibitor (SBTI) and the human recombinant α₁-AT mutant, α₁-AT-Arg³⁵⁸ were in the order: α₁-AT - TPCK> α₁-AT-Arg³⁵⁸ > SBTI when cells were stimulated with concanavalin A plus cytochalasin E. These data suggest that, in human inflammatory fluks containing relatively high concentrations of α₁-AT (such as rheumatoid arthritis synovial fluids) (i) α₁-AT may down-regulate the inflammatory process by inhibiting the neurophil respiratory burst and (ii) serpin oxidation by neutrophil-released oxygen species is unlikely to occur.

【 授权许可】

Unknown