FEBS Letters | |
Subunit communication in the tryptophan synthase α2β2 complex Effects of β subunit ligands on proteolytic cleavage of a flexible loop in the α subunit | |
Miles, Edith Wilson1  Ruvinov, Sergei B.1  | |
[1] Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 8, Room 225, Bethesda, MD 20892, USA | |
关键词: Tryptophan synthase; Subunit communication; Allosteric mechanism; Protein loop; Proteolysis; Ligand binding; | |
DOI : 10.1016/0014-5793(92)80246-D | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To probe the structural basis for ligand-mediated communication between the α and β subunits in the tryptophan synthase α2β2 complex, we have determined the effects of ligands of the α and β subunits on proteolysis of a flexible loop in the α subunit. We find that addition of a ligand of the β subunit (l-serine, d-tryptophan, or l-tryptophan) in combination with a ligand of the α subunit (α-glycerol 3-phosphate) almost completely prevents the tryptic cleavage of the α subunit loop. Thus, the binding of a ligand to the β-site affects the conformation of the α subunit 25–30 Å distant.
【 授权许可】
Unknown
【 预 览 】
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