Inhibitory effect of oryzacystatins and a truncation mutant on the replication of poliovirus in infected Vero cells

FEBS Letters

Arai, Soichi¹  Abe, Keiko¹  Ijiri, Sumiko²  Kondo, Hiroto¹  Maeda, Hiroshi² 

[1] Department of Agricultural Chemistry, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Department of Microbiology, Kumamoto University Medical School, Honjo, Kumamoto 860, Japan

关键词: Oryzacystatin;    Poliovirus;    Rice seed;    Cysteine proteinase inhibitor;    Cystatin superfamily;    Anti-viral effect;   

DOI  :  10.1016/0014-5793(92)80097-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Poliovirus, a picornavirus family member, requires the processing of its poly-protein by its own cysteine proteinase for replication. Oryzacystatin-I and oryzacystatin-II, proteinaceous cysteine proteinase inhibitors (cystatins) of rice seed origin, were found to inhibit the replication of poliovirus effectively in infected Vero cells in vitro. Truncated oryzacystatin-I, which lacks the NH₂-terminal 25 amino acid residues of the intact protein, is an even more effective inhibitor, eliciting its effect at concentrations of less than 0.23 nmol/ml. The low molecular weight cysteine proteinase inhibitors, E-64, E-64C and loxistatin, showed no anti-viral effect at any concentration investigated.

【 授权许可】

Unknown