Mammalian expression of the human sex steroid‐binding protein of plasma (SBP or SHBG) and testis (ABP) Characterization of the recombinant protein

FEBS Letters

Sui, Li-ming³  Hagen, Frederick S.²  Arguelles, Cesar³  Zhang, Wei³  Seidel, Paul R.³  Petra, Philip H.³  Conroy, Stephen C.¹ 

[1] Biochemistry, University of Washington, Seattle, WA 98195, USA;Zymogenetics Inc., Seattle, WA 98105, USA;Departments of Obstetrics and Gynecology Washington, Seattle, WA 98195, USA

关键词: Sex steroid-binding protein;    SBP;    SHBG;    Mammalian expression;   

DOI  :  10.1016/0014-5793(92)80091-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A full-length 1,209 bp cDNA encoding the human sex steroid-binding protein of plasma (SBP or SHBG) and testis (ABP) was constructed and expressed in BHK-21 cells. The sequence agrees with the published gene and protein sequences. The cells were found to secrete SBP following transfection and G418^r selection. The recombinant protein binds 5α-dihydrotestosterone with a K _d of 0.28 nM. It also binds testosterone and 17β-estradiol but not progesterone, estrone or cortisol revealing a steroid-binding specificity identical to that of human SBP, SDS-PAGE patterns are less complex than human SBP and show a monomeric molecular weight of about 43 kDa.

【 授权许可】

Unknown