High protein mobility in skinned rabbit muscle fibres observed by 1H NMR spectroscopy

FEBS Letters

Kalbitzer, Hans Robert¹  Wray, John¹  Schrumpf, Matthias¹ 

[1] Max-Planck-Institute for Medical Research, Department of Biophysics, Jahnstr. 29, D-6900 Heidelberg, Germany

关键词: Muscle;    Myosin;    NMR;    Skinned fibres;    Mobility;    DSS;    4;    4-dimethyl-4-silapentane-sulphonic acid;    EGTA;    ethylene glycol bis(β-aminoethyl ether) N;    NN′;    N′-tetraacetic acid;    MOPS;    3-(N-morpholino)propane sulphonic acid;    NMR;    nuclear magnetic resonance;   

DOI  :  10.1016/0014-5793(92)80063-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

¹H NMR spectra of skinned rabbit muscle fibers show a group of relatively sharp resonance lines which presumably originate from highly mobile protein domains. Comparison with the spectrum of myosin subfragment I suggests that these signals may come at least partly from mobile regions of the myosin head. NMR could possibly be used to characterize the movements of crossbridges in force generation.

【 授权许可】

Unknown