Immunoreactive thyroliberin (TRH) precursor forms in human hypothalamus and anterior pituitary tissues

FEBS Letters

Pagésy, P.²  Peillon, F.²  Grouselle, D.¹  Croissandeau, G.²  Le Dafniet, M.²  Li, J.Y.² 

[1] Groupe de Neuroendocrinologie Cellulaire, Collège de France, 11 Rue Marcelin-Berthelot, 75005 Paris, France;INSERM U223, Faculté de Médecine Pitié-Salpêtrière, 105 Boulevard de l'Hôpital, 75013, Paris, France

关键词: TRH;    Precursor;    Human hypothalamus and anterior pituitary;   

DOI  :  10.1016/0014-5793(92)80054-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Immunoreactive (IR) proTRH forms were characterized in human hypothalamic tissue with two antisera raised against a hepta- and a decapeptide containing the TRH progenitor sequence (-Gln-His-Pro-Gly-). A similar study was performed in human normal and adenomatous anterior pituitaries, tissues in which TRH synthesis has been previously suggested. IR-proTRH was found in all the samples ranging from 42–775 fmol/mg proteins. Size exclusion chromatography identified a major 25–35 kDa form and a minor 4–8 kDa form. The existence of the major form was confirmed by immunoblotting. The results suggest that both human hypothalamic and normal or adenomatous anterior pituitary tissues synthesize similar IR-proTRH forms.

【 授权许可】

Unknown