17O NMR and FT‐IR study of the ionization state of peptides in aprotic solvents Application to Leu‐enkephalin

FEBS Letters

Vitoux, B.¹  Sakarellos-Daitsiotis, M.²  Sakarellos, C.²  Tsikaris, V.²  Karayannis, T.²  Birlirakis, N.²  Gerothanassis, I.P.²  Marraud, Michel¹ 

[1] Laboratory of Macromolecular Physical Chemistry, URA-CNRS-494, ENSIC-INPL, BP 451, 54001 Nancy Cedex, France;Department of Chemistry, University of Ioannina, PO Box 1186, 45110 Ioannina, Greece

关键词: FT-IR;    Ionization state of peptide;    Leu-enkephalin;    17O NMR;   

DOI  :  10.1016/0014-5793(92)80053-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ionization state of Leu-enkephalin in DMSO and MeCN/DMSO (4/1) solution was studied by the combined use of ¹⁷O NMR and FT-IR spectroscopy. After lyophilization or an aqueous solution at nearly neutral pH, Leu-enkephalin essentially exists in the uncharged state in MeCN/DMSO (4/1) solution. In pure DMSO, only 40% of the Leu-enkephalin molecules are in the zwitterionic state under the same conditions.

【 授权许可】

Unknown