Concentration‐dependent modulation of basic fibroblast growth factor action on multiplication and locomotion of human teratocarcinoma cells

FEBS Letters

Ektröm, Tomas J.¹  Granerus, Marika¹  Schofield, Paul N.²  Engström, Wilhelm¹  Lee, Amanda² 

[1] Center for Biotechnology, Karolinska Institutet, NOVUM, S-14152 Huddinge, Sweden;Department of Anatomy, University of Cambridge, Downing street, Cambridge, CB2 3DY, UK

关键词: Fibroblast growth factor;    Cell multiplication;    Cell motility;   

DOI  :  10.1016/0014-5793(92)80044-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1–10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamin sulphate. When high concentrations of bFGF were added, a preferential effect on cell locomotion was observed. 100 ng bFGF/ml stimulated cell movement but only exerted a marginal effect on cell multiplication. These observed exogenous requirements for multiplication and locomotion were complemented by the expression of bFGF receptors. Scatchard analysis of binding data suggests the existence of a high-affinity and a low-affinity class of receptors.

【 授权许可】

Unknown