Superproduction of heavy minicircular mitochondrial DNA in aging wheat coleoptiles

FEBS Letters

Kirnos, M.D.¹  Vanyushin, B.F.¹  Alexandrushkina, N.I.¹  Zagorskaya, G.Ya.¹  Kireev, I.I.¹ 

[1] A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119899 USSR

关键词: Heavy wheat mitochondrial DNA;    Synthesis Size;    mtDNA;    mitochondrial DNA;    H-mtDNA;    heavy mitochondrial DNA;    nDNA;    nuclear DNA;   

DOI  :  10.1016/0014-5793(92)80033-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

On incubation of 7-day-old wheat seedlings in the presence of [³H]thymidine, the radioactivity incorporated into coleoptile DNA is found to be localized mainly (>95%) in the fraction of heavy mitochondrial DNA (H-mt DNA: ϱ = 1.716 gm/cm³). Upon long (48–72 h) incubation of cut-off seedlings in water, the amount of this DNA shows a dramatic increase corresponds to about 10% of the total coleoptile DNA. H-mtDNA is represented by open circular molecules with a contour length varying from 0.12 to 0.6μm. The functional role of this DNA is still unknown.

【 授权许可】

Unknown