31P NMR investigation of the backbone conformation and dynamics of the hexamer duplex d(5′‐GCATGC)2 in its complex with the antibiotic nogalamycin

FEBS Letters

Lane, Andrew N.²  Searle, Mark S.¹ 

[1] Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK;Laboratory of Molecular Structure, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK

关键词: 31P NMR relaxation;    (Nogalamycin)2/d(GCATGC)2;    Chemical shift anisotropy;    Correlation time;    1H−31P coupling;   

DOI  :  10.1016/0014-5793(92)80558-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heteronuclear chemical shift correlation experiments confirm that the two down-field shifted ³¹P resonances in the spectrum of the (nogalamycin)₂-d(GCATGC)₂ complex correspond to the phosphodiesters CpA and TpG at the intercalation sites. ³¹P relaxation measurements (R₁, R₂ and {¹H}-³¹P NOE) at 4.7 and 9.4 T permit the correlation time of each phosphate to be determined together with their chemical shift anisotropies. Significant differences in deoxyribose H3′−³¹P coupling constants and chemical shift anisotropy contributions are observed, consistent with an asymmetric DNA backbone conformation for the phosphate groups at the intercalation sites. Large amplitude internal motions of the phosphates do not appear to contribute significantly to relaxation.

【 授权许可】

Unknown