Hepatocyte growth factor (HGF) mediates the sustained formation of 1,2‐diacylglycerol via phosphatidylcholine—phospholipase C in cultured rat hepatocytes

FEBS Letters

Nakashima, Shigeru²  Saji, Shigetoyo¹  Nozawa, Yoshinori²  Osada, Shinji¹  Nakamura, Toshikazu³ 

[1] The Second Department of Surgery, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan;Department of Biochemistry, Gifu University School of Medicine, Tsukasamachi-40, Gifu 500, Japan;Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812, Japan

关键词: Hepatocyte growth factor (HGF);    Sustained DG formation;    Phosphatidylcholine-phospholipase C;    Ca2+ oscillation;   

DOI  :  10.1016/0014-5793(92)80554-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The addition of hepatocyte growth factor (HGF) to rat hepatocytes in primary culture resulted in the formation of inositol 1,4,5-trisphosphate (Ins(1,4,5)P₃) and 1,2-diacylglycerol (DG) by a phosphoinositide-specific phospholipase C (PI-PLC). DG showed a biphasic increase; the first phase, corresponding with the peak of Ins(1,4,5)P₃ and a second larger and prolonged phase. The HGF stimulates the phosphatidylcholine (PC)-derived prolonged DG formation by a phospholipase C pathway (PC-PLC) but not by a phospholipase D pathway. HGF also was found to elicit [Ca²⁺] oscillations which may be associated with the prolonged DG production from PC via the PC-PLC phospholipase C pathway.

【 授权许可】

Unknown