A novel 15N‐labeling method to selectively observe 15NH2 resonances of proteins in 1H‐detected heteronuclear correlation spectroscopy

FEBS Letters

Tate, Naoko U.²  Ravera, Mark W.¹  Jaye, Michael¹  Inagaki, Fuyuhiko²  Tate, Shin-ichi² 

[1] Rhone-Poulenc Rorer Central Research, 660 Allendale Road, King of Prussia, PA 19406, USA;Department of Molecular Physiology, The Tokyo Metropolitan Institute of Medical Science, Honkomagome 3-chome, Bunkyo-ku, Tokyo 113, Japan

关键词: 1H-detected heteronuclear correlation spectroscopy;    15N-labeling;    Acidic fibroblast growth factor;    HMQC;    heteronuclear multiple quantum coherence;    HSQC;    heteronuclear single quantum coherence;    DEPT;    distortionless enhancement of polarization transfer;    aFGF;    acidic fibroblast growth factor;   

DOI  :  10.1016/0014-5793(92)80322-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An experimental method to selectively label side-chain NH₂ groups of glutamine and asparagine in proteins with ¹⁵N is proposed. This selective labeling method enables to observe only ¹⁵NH₂ resonances and thus, to discriminate between ¹⁵NH and ¹⁵NH₂ resonances in a ¹H-detected heteronuclear correlation spectrum. This method gives results with approximately two times higher sensitivity than those obtained by elaborate pulse sequences such as DEPT-HSQC and will be useful for studying the molecular interaction involving the side chains of Asn and Gln residues.

【 授权许可】

Unknown