A plant homologue to mammalian brain 14‐3‐3 protein and protein kinase C inhibitor

FEBS Letters

Hirsch, Stephan²  Soll, Jürgen²  Bertsch, Uwe³  Aitken, Alastair¹ 

[1] National Institute for Medical Research, Mill Hill, London NW7 1AA, England;Botanisches Institut, Universität Kiel, Olshausenstraße 40, 2300 Kiel, Germany;Fachrichtung Botanik, Universität Saarbrücken, 6600 Saarbrücken, Germany

关键词: Protein phosphorylation;    14-3-3 protein;    Protein kinase C inhibitor;    PKC;    protein kinase C;    KCIP;    inhibitor protein of PKC;   

DOI  :  10.1016/0014-5793(92)80384-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated cDNA clones of Spinacea oleracea L. and Oenothera hookeri of 930 and 1017 base pairs, respectively. The open reading frame deduced from the Oenothera sequence codes for a protein of a calculated molecular mass of 29 200. The primary amino acid sequence exhibits a very high degree (88%) of homology to the 14-3-3 protein from bovine brain, and protein kinase C inhibitor from sheep brain. Subsequently the plant protein was partially purified from leaf extract. The partially purified plant protein inhibited protein kinase C from sheep brain in a heterologous assay system. The active fraction consisted of 5–6 different polypeptides of similar molecular size. One of these proteins crossreacted with a peptide-specific antibody against protein kinase C inhibitor protein from sheep brain.

【 授权许可】

Unknown