Biotinyl analogues of amylin as biologically active probes for amylin/CGRP receptor recognition

FEBS Letters

Day, Anthony J.²  Foot, Elizabeth A.¹  Willis, Antony C.²  Leighton, Brendan¹  Chantry, Andrew² 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK;MRC Immunochemistry Unit, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

关键词: Amylin;    CGRP;    Receptor;    Biotinylation;    Non-insulin-dependent diabetes mellitus;   

DOI  :  10.1016/0014-5793(92)80362-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Biotinyl analogues of rat amylin were synthesised with sulfosuccinimidyl 2-(biotinamido)ethyl-1,3-dithiopropionate(NHS-SS-Biotin). Biotinylated amylin peptides were purified by HPLC, quantitated, and the presence of the biotin group at Lys-1 confirmed by peroxidase-labelled avidin and FAB mass spectroscopy. Amylin-biotin retained a similar affinity for binding to rat liver plasma membranes compared with rat amylin and also completely inhibited insulin-stimulated glycogen synthesis in rat soleus muscle incubated in vitro. These biologically active amylin probes will enable a complete analysis of amylin/CGRP receptor expression in various cell types and facilitate the isolation and characterisation of the hormone-receptor complex.

【 授权许可】

Unknown