Production of inositol phosphates and reactive oxygen metabolites in quartz‐dust‐stimulated human polymorphonuclear leukocytes

FEBS Letters

Savolainen, Kai M.¹  Tuomala, Marjo H.¹  Hirvonen, Maija-Riita¹ 

[1] National Public Health Institute, Department of Environmental Hygiene and Toxicology, SF-70701 Kuopio, Finland

关键词: Quartz;    Inositol phosphate;    Reactive oxygen metabolite;    Polymorphonuclear leukocyte;    HPLC;    high performance liquid chromatography;    PIP2;    phosphatidyl-inositol 4;    5-bisphosphate;    DAG;    diacylglycerol;    InsP1;    inositol monophosphates;    InsP2;    inositol 1;    4-bisphosphate;    InsP3;    inositol trisphosphates;    InsP4;    inositol 1;    3;    4;    5-tetrakisphosphate;    PKC;    protein kinase C;    TBAP;    tetrabutyl ammonium phosphate;    PMNL;    polymorphonuclear leukocytes;    CL;    chemiluminescence;   

DOI  :  10.1016/0014-5793(92)80402-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The present paper explores phosphoinositide turnover in quartz-stimulated human polymorphonuclear leukocytes. Separation of inositol phosphates was carried out with a new ion-pair, reverse-phase high performance liquid chromatographic method applying a gentle tetrabutylammonium phosphate buffer gradient. The method separates inositol monophosphates, inositol 1,4-bisphosphate, inositol trisphosphates and inositol 1,3,4,5-tetrakisphosphate. Reactive oxygen metabolites, indices for leukocyte activation, were measured with a luminometric assay. Quartz increased the production of reactive oxygen metabolites, preceded by facilitated inositol phosphate turnover. This finding provides evidence that inositol phosphate second messengers may be involved in quartz-induced leukocyte activation and subsequent production of reactive oxygen metabolites.

【 授权许可】

Unknown