Phosphorylation‐activated 6‐phosphofructo‐2‐kinase from mantle tissue of marine mussels

FEBS Letters

Ramos-Martinez, J.I.¹  Vázquez-Illanes, M.D.¹ 

[1] Departamento de Bioquímica y Biología Molecular, Universidad de Santiago de Compostela, Facultad de Veterinaria, E-27002 Lugo, Spain

关键词: 6-Phosphofructo-2-kinase;    Phosphorylation;    Molluse;    Mantle tissue;    Mytilus galloprovincialis Lmk;    Fru-1;    6-P2;    fructose-1;    6-bisphosphate;    Fru-6-P;    fructose-6-phosphate;    Fru-2;    6-P2;    fructose-2;    6-bisphosphate;    FBPase-1;    fructose-1;    6-bisphosphatase (EC 3.1.3.11);    PFK-1;    6-phosphofructo-1-kinase (EC 2.7.1.11);    PFK-2;    6-phosphofructo-2-kinase (EC 2.7.1.11);    PEP;    phosphoenolpyruvate;   

DOI  :  10.1016/0014-5793(91)81412-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PKF-2 from mussel mantle was phosphorylated by cAMP-dependent protein kinase. The phosphorylation does not change the enzyme activity at neutral pH values, but at acid pH the activity of the phosphorylated form is higher than the native PFK-2. With respect to the native enzyme, the activation consisted of a reduction in the K _fm for Fru-6-P and a decrease in the inhibitory effect of PEP. These results are in keeping with the stabilized concentration of Fru-2,6-P₂ found in the mussel mantle during the physiological hypoxia caused by the closure of the valves.

【 授权许可】

Unknown