Alternatively spliced mRNA of the pX region of human T lymphotropic virus type I proviral genome

FEBS Letters

Furukawa, Keiko¹  Furukawa, Koichi¹  Shiku, Hiroshi¹ 

[1] Department of Oncology, Nagasaki University School of Medicine, 12-4, Sakamoto-machi, Nagasaki 852, Japan

关键词: HTLV-I;    tax Gene;    rex Gene;    pX Protein;    Alternative splicing;    HTLV-I;    human T lymphotropic virus type I;    ATL;    adult T cell leukemia;    HAM/TSP;    HTLV-I-associated myelopathy/tropical spastic paraparesis;    PBMC;    peripheral blood mononuclear cells;    RT;    reverse transcription;    PCR;    polymerase chain reaction;    ECL;    enhanced chemiluminescence;   

DOI  :  10.1016/0014-5793(91)81404-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The pX region of human T lymphotropic virus type I (HTLV-1) is believed to be expressed as a consequence of a 2 step splicing. It is conceivable, however, that a donor site of the 1st splicing and an acceptor site of the 2nd splicing results in the production of an alternatively spliced mRNA which is capable of coding p21^X-III. This possibility was examined by amplifying cDNA derived from HTLV-I⁺ cells between the 5′ LTR and pX region. Bands of 2 different sizes were consistently observed. Sequencing of the longer band corresponded to a cDNA derived from a double-spliced pX mRNA as previously reported. The shorter band was derived from a single-spliced mRNA. HTLV-I⁺ cell lines had both mRNAs to a varying degree. Expression of p40^tax and p21^X-III seem to be well correlated with a double-spliced and a single-spliced mRNA, respectively.

【 授权许可】

Unknown